|  e-ISSN: 2757-6620

Original article | Journal of Agricultural Production 2021, Vol. 2(2) 44-47

Abundance of Culturable Marine Heterotrophic Bacteria in Ulva lactuca Associated with Farmed Seaweeds Kappaphycus spp. and Eucheuma denticulatum

Albaris B. Tahiluddin, Tadzmahal I. Alawi, Nurul Syakila A. Hassan, Sitti Nurhaliza A. Jaji, Ertugrul Terzi

pp. 44 - 47   |  DOI: https://doi.org/10.29329/agripro.2021.360.1   |  Manu. Number: MANU-2109-30-0001.R1

Published online: December 03, 2021  |   Number of Views: 56  |  Number of Download: 215


Abstract

Ulva lactuca is one of the macro-epiphytes of farmed seaweeds Kappaphycus spp. and Eucheuma denticulatum in Tawi-Tawi, southern Philippines, especially during the summer season, which affects the growth and health of farmed seaweeds. In this study, the abundance of culturable heterotrophic marine bacteria from U. lactuca associated with farmed seaweeds Kappaphycus spp. and E. denticulatum was investigated in the seaweed farms of Tongsibalo, Sibutu, Tawi-Tawi, southern Philippines, using serial dilution procedure. Results revealed that the average bacterial counts obtained from U. lactuca associated with Kappaphycus alvarezii, K. striatus, and E. denticulatum were 2.48 x 1010 CFU g-1, 1.14 x 1012 CFU g-1, and 1.32 x 1011 CFU g-1, respectively. In addition, agar-digesting bacteria were observed from U. lactuca samples associated with K. alvarezii and K. striatus manifested by the depression and liquefaction of the marine agar after 2-3 days which were suspected as pathogenic bacteria causing ice-ice disease. Therefore, U. lactuca may serve as a vector for these potential pathogens to farmed seaweeds.

Keywords: Seaweed, Heterotrophic marine bacteria, Eucheuma denticulatum, Kappaphycus, Ulva lactuca


How to Cite this Article?

APA 6th edition
Tahiluddin, A.B., Alawi, T.I., Hassan, N.S.A., Jaji, S.N.A. & Terzi, E. (2021). Abundance of Culturable Marine Heterotrophic Bacteria in Ulva lactuca Associated with Farmed Seaweeds Kappaphycus spp. and Eucheuma denticulatum . Journal of Agricultural Production, 2(2), 44-47. doi: 10.29329/agripro.2021.360.1

Harvard
Tahiluddin, A., Alawi, T., Hassan, N., Jaji, S. and Terzi, E. (2021). Abundance of Culturable Marine Heterotrophic Bacteria in Ulva lactuca Associated with Farmed Seaweeds Kappaphycus spp. and Eucheuma denticulatum . Journal of Agricultural Production, 2(2), pp. 44-47.

Chicago 16th edition
Tahiluddin, Albaris B., Tadzmahal I. Alawi, Nurul Syakila A. Hassan, Sitti Nurhaliza A. Jaji and Ertugrul Terzi (2021). "Abundance of Culturable Marine Heterotrophic Bacteria in Ulva lactuca Associated with Farmed Seaweeds Kappaphycus spp. and Eucheuma denticulatum ". Journal of Agricultural Production 2 (2):44-47. doi:10.29329/agripro.2021.360.1.

References

    Albakosh, M. A., Naido, R. K., Kirby, B., & Bauer, R. (2016). Identification of epiphytic bacterial communities associated with the brown alga Splachnidium rugosum.  Journal of Applied Phycology, 28, 1891-1901. https://doi.org/10.1007/s10811-015-0725-z

    Armstrong, E., Rogerson, A., & Leftley, J. W. (2000). Utilisation of seaweed carbon by three surface-associated heterotrophic protists, Stereomyxa ramosa, Nitzschia alba and Labyrinthula sp. Aquatic Microbial Ecology, 21, 49-57. https://doi.org/10.3354/ame021049

    Chellaram, C., Raja, P., John, A. A., & Krithika, S. (2013). Antagonistic effect of epiphytic bacteria from marine algae, southeastern India. Pakistan journal of biological sciences: PJBS16(9), 431-434. https://doi.org/10.3923/pjbs.2013.431.434

    FDA. (2001). Bacteriological analytical manual chapter 3: Aerobic plate count. Retrieved March 20, 2019, from https://www.fda.gov/Food/FoodScienceReacher/LaboratoryMethods/ucm06 3346.htm

    Largo, D. B. (2002). Recent developments in seaweeds diseases. In A. Q. Hurtado, N. G. Guanzone Jr., T. R. de Castro- Mallare & M. R. J. Luhan (Eds.), Proceedings of the National Seaweed Planting Work Shop held on August 2-3, 2001 (pp. 35-42). SEAFDEC Aquaculture Department.

    Largo, D. B., Fukami, K., & Nishijima, T. (1995a). Occasional pathogenic bacteria promoting ice-ice disease in the carrageenan-producing red algae Kappaphycus alvarezii and Eucheuma denticulatum (Solieriaceae, Gigartinales, Rhodophyta). Journal of Applied Phycology, 7, 545-554. https://doi.org/10.1007/BF00003941

    Largo, D. B., Fukami, K., & Nishijima, T. (1999). Time-dependent attachment mechanism of bacterial pathogen during “ice-ice” infection in Kappaphycus alvarezii (Gigartinales, Rhodophyta). Journal of Applied Phycology, 11, 129-136. https://doi.org/10.1023/A:1008081513451

    Largo, D. B., Fukami, K., Nishijima, T., & Ohno, M. (1995b). Laboratory-induced development of the ice-ice disease of the farmed red algae Kappaphycus alvarezii and Eucheuma denticulatum (Solieriaceae, Gigartinales, Rhodophyta). Journal of Applied Phycology7, 539-543. https://doi.org/10.1007/BF00003940

    López-Gappa, J. J., Tablado, A., & Magaldi, N. H. (1990). Influence of sewage pollution on a rocky intertidal community dominated by the mytilid Brachidontes rodriguezi. Marine Ecology Progress Series, 63, 163-175. https://doi.org/10.3354/meps063163

    Martinez, J. N., & Padilla, P. I. P. (2016). Isolation and characterization of agar-digesting Vibrio species from the rotten thallus of Gracilariopsis heteroclada Zhang et Xia. Marine Environmental Research119, 156-160. https://doi.org/10.1016/j.marenvres.2016.05.023

    Pang, T., Liu, J., Liu, Q., Li, H., & Li, J. (2015). Observations on pests and diseases affecting a eucheumatoid farm in China. Journal of Applied Phycology27, 1975-1984. https://doi.org/10.1007/s10811-014-0507-z

    Singh, R. P., & Reddy, C. R. K. (2014). Seaweed–microbial interactions: key functions of seaweed-associated bacteria. FEMS Microbiology Ecology88(2), 213-230. https://doi.org/10.1111/1574-6941.12297

    Tahiluddin, A. & Terzi, E. (2021b). An overview of fisheries and aquaculture in the Philippines. Journal of Anatolian Environmental and Animal Sciences, 6(4), 475-486. https://doi.org/10.35229/jaes.944292

    Tahiluddin, A. B. (2018). Influence of fertilization on the occurrence of Vibrio, “ice-ice” disease and growth of seaweeds Kappaphycus striatus (F. Schmitz) Doty ex P.C. Silva. (Master’s thesis, University of the Philippines Visayas).

    Tahiluddin, A. B., & Terzi, E. (2021a). Ice-ice disease in commercially cultivated seaweeds Kappaphycus spp. and Eucheuma spp.: A review on the causes, occurrence, and control measures. Marine Science and Technology Bulletin, 10(3), 234-243. https://doi.org/10.33714/masteb.917788

    Tahiluddin, A. B., Nuñal, S. N., Luhan, M. R. J., & Santander–de Leon, S. M. S. (2021). Vibrio and heterotrophic marine bacteria composition and abundance in nutrient-enriched Kappaphycus striatus. Philippine Journal of Science, 150(6B), 1549-1761.

    Teeling, H., Fuchs, B. M., Becher, D., Klockow, C., Gardebrecht, A., Bennke, C. M., & Amann, R. (2012). Substrate-controlled succession of marine bacterioplankton populations induced by a phytoplankton bloom. Science336(6081), 608-611.

    Yulianto, K., & Mira, S. (2009). Macro algae cultivation of Kappaphycus alvarezii (Linn., 1758) vertically and symptoms of “ice-ice” disease in the Water of Pari Island, Pulau Pari. Oceanology and Limnology Journal, 35(3), 323-332.